Psychedelic-Inspired Drug Discovery Using an Engineered Biosensor. Dong, Chunyang and Ly, Calvin and Dunlap, Lee E. and Vargas, Maxemiliano V. and Sun, Junqing and Hwang, In-Wook and Azinfar, Arya and Oh, Won Chan and Wetsel, William C. and Olson, David E. and Tian, Lin, Available


Cell-type-specific asynchronous modulation of PKA by dopamine in learning. Lee SJ, Lodder B, Chen Y, Patriarchi T, Tian L, Sabatini BL. Nature. 2020 Dec 23. doi: 10.1038/s41586-020-03050-5. Epub ahead of print. PMID: 33361810.

Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning. Unger EK, Keller JP, Altermatt M, Liang R, Matsui A, Dong C, Hon OJ, Yao Z, Sun J, Banala S, Flanigan ME, Jaffe DA, Hartanto S, Carlen J, Mizuno GO, Borden PM, Shivange AV, Cameron LP, Sinning S, Underhill SM, Olson DE, Amara SG, Temple Lang D, Rudnick G, Marvin JS, Lavis LD, Lester HA, Alvarez VA, Fisher AJ, Prescher JA, Kash TL, Yarov-Yarovoy V, Gradinaru V, Looger LL, Tian L. CELL. 2020 Dec 23;183(7):1986-2002.e26. doi: 10.1016/j.cell.2020.11.040. Epub 2020 Dec 16. PMID: 33333022.

Imaging Neurotransmitter and Neuromodulator Dynamics In Vivo with Genetically Encoded Indicators. Sabatini BL, Tian L. Neuron. 2020 Oct 14;108(1):17-32. doi: 10.1016/j.neuron.2020.09.036. PMID: 33058762.

An expanded palette of dopamine sensors for multiplex imaging in vivo. Patriarchi T, Mohebi A, Sun J, Marley A, Liang R, Dong C, Puhger K, Mizuno GO, Davis CM, Wiltgen B, von Zastrow M, Berke JD, Tian L. Nat Methods. 2020 Nov;17(11):1147-1155. doi: 10.1038/s41592-020-0936-3. Epub 2020 Sep 7. PMID: 32895537.

Imaging voltage and brain chemistry with genetically encoded sensors and modulators. Pal A, Tian L. Curr Opin Chem Biol. 2020;57:166-176.


Cell-type specific asynchronous modulation of PKA by dopamine during reward based learning, Lee SJ, Lodder B, Chen Y, Patriarchi T, Tian L, Sabatini B. BioRxiv, Nov 12, 2019

Optical dopamine monitoring with dLight1 reveals mesolimbic phenotypes in a mouse model of neurofibromatosis type 1. Robinson JE, Coughlin GM, Hori AM, Cho JR, Mackey ED, Turan Z, Patriarchi T, Tian L, Gradinaru V. Elife. 2019 Sep 23;8. doi: 10.7554/eLife.48983.

Imaging neuromodulators with high spatiotemporal resolution using genetically encoded indicators, Patriarchi T, Cho JR, Merten K, Marley A, Broussard GJ, Liang R, von Zastrow M, Nimmerjahn A, Gradinaru V, Williams JT, Tian L. Nature Protocols. 14: 3471–3505(2019)

Measuring brain chemistry using genetically encoded fluorescent sensors, Andreoni A, Davis C and Tian L. Current opinion in Biomedical Engineering, Vol 12:59-67, Dec 2019

A Neural Circuit Mechanism for Encoding Aversive Stimuli in the Mesolimbic Dopamine System. de Jong JW, Afjei SA, Pollak Dorocic I, Peck JR, Liu C, Kim CK, Tian L, Deisseroth K, Lammel S. Neuron. 2018 Nov 19.

Dissociable dopamine dynamics for learning and motivation. Mohebi A, Pettibone JR, Hamid AA, Wong JT, Vinson LT, Patriarchi T, Tian L, Kennedy RT, Berke JD. Nature. 2019 Jun;570(7759):65-70. doi: 10.1038/s41586-019-1235-y. Epub 2019 May 22. PubMed PMID: 31118513; PubMed Central PMCID: PMC6555489.

Maps of neuronal activity across the mouse brain. Alessio Andreoni and Lin Tian. Nat Biomed Eng. 2019 May;3(5):335-336.

Protein structures guide the design of a much-need tool for neuroscience. Scheerer P, Unger E and Tian L. Nature, 2018 Sep: 561(7723):312-313.

Real-time monitoring of neuromodulators in behaving animals using genetically encoded indicators. Grace O. Mizuno, Elizabeth Unger and Lin Tian. Book chapter, Compendium of In-Vivo monitoring in Real-Time Molecular Neuroscience, vol3, 2019.

Temporally and Spatially Distinct Thirst Satiation Signals. Augustine V, Ebisu H, Zhao Y, Lee S, Ho B, Mizuno GO, Tian L, Oka Y. Neuron. 2019 Jul 17;103(2):242-249.e4. doi: 10.1016/j.neuron.2019.04.039. Epub 2019 May 29. PubMed PMID: 31153646.


Imaging glutamate with genetically encoded fluorescent sensors,Broussard G, Unger L, Liang R, Tian L, book chapter, Biochemical approaches for glutamatergic neurotransmission, 117-153, 2018

Aberrant Calcium Signaling in Astrocytes Inhibits Neuronal Excitability in a Human Down Syndrome Stem Cell Model. Mizuno GO, Wang Y, Shi G, Wang Y, Sun J, Papadopoulos S, Broussard GJ, Unger EK, Deng W, Weick J, Bhattacharyya A, Chen CY, Yu G, Looger LL, Tian L. Cell Rep. 2018 Jul 10;24(2):355-365.

Dopamine neurons projecting to medial shell of the nucleus accumbens drive heroin reinforcement. Corre J, van Zessen R, Loureiro M, Patriarchi T, Tian L, Pascoli V, Lüscher C. Elife. 2018 Oct 30;7.

In vivo measurement of afferent activity with axon-specific calcium imaging. Broussard G, Liang R, Fridman M, Unger E, Meng GH, Xian X, Ji N, Petreanu L, Tian L, Nature Neuroscience, 21, 1272, 2018.

Nanodelivery of a functional membrane receptor to manipulate cellular phenotype. Patriarchi T, Shen A, He W, Baikoghli M, Cheng R., Xiang Y, Coleman M, Tian L, Scientific Reports 8, 2018

Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors. Patriarchi T, Cho JR, Merten K, Howe MW, Marley A, Xiong WH, Folk RW, Broussard GJ, Liang R, Jang MJ, Zhong H, Dombeck D, von Zastrow M, Nimmerjahn A, Gradinaru V, Williams JT, Tian L. Science. 2018 Jun 29;360(6396). Recommend by F1000.   


Combinatorial Library Screening with Liposomes for Discovery of Membrane Active Peptides. C. Randy, T. Yann, K. Zsofia, S. Amir, H. Campos, K. Alisha, R. Liu, L. Tian, K. Lam, ACS Combinatorial Science 19 (5),  (2017).


Automated Functional Analysis of Astrocytes from Chronic Time-Lapse Calcium Imaging Data. Wang Y, Shi G, Miller DJ, Wang Y, Wang C, Broussard G, Wang Y, Tian L, Yu G. Front Neuroinform. 2017 Jul 14;11:48.

Functional imaging of neuron-astrocyte interactions in a compartmentalized microfluidic device. Y. Gao, G. Broussard, A. Haque, A. Revzin, and L. Tian, Nature: Microsystems & Nanoengineering 2, (2016).

Graphical Time Warping for Joint Alighment of Multiple Curves. Y. Zhi, G. Shi, D. J. Miller, G. Broussard, L. Tian*, and G. Yu, paper 1815, Neural Information Processing Systems, 2016

Wnt regulates proliferation and neurogenic potential of Muller Glial cells through a Lin28/let-7 miRNA-dependent pathway in adult mammalian retina. K. Yao, S. Qiu, L. Tian, W. Snider, J. Flannery, D. Scahffer, B. Chen, Cell Rep. 17, 2016.


CDK1 enhances mitochondrial bioenergetics for radiation-induced DNA repair. L. Qin, M. Fan, D. Candas, G. Jiang, S. Papadopoulos. L. Tian, G. Woloschak, D. J. Grdina, and J. J. Li, Cell reports 13, 2056-2063 (2015).

Imaging chemical neurotransmission with genetically encoded fluorescent sensors. R. Liang, G. Broussard, and L. Tian, ACS chemical neuroscience 6, 84-93 (2015).


Monitoring activity in neural circuits with genetically encoded indicators. G. Broussard, R. Liang, & L. Tian, Frontiers in molecular neuroscience 7, 97 (2014).


An optimized fluorescent probe for visualizing glutamate neurotransmission. J. Marvin, B. G. Borghuis, L. Tian et al. Nature methods 10, 162-170 (2013).

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics. J. Akerboom, N. Carreras Calderon, L. Tian et al, Frontiers in molecular neuroscience 6, 2 (2013).

Two Photon Photophysics of Fluorescent Protein Calcium Indicators. J. Macklin, J. Akerboom, E. R. Schreiter, L. Tian, R. Patel, V. Iyer, B. Karsh, J. Colonell, and T. D. Harris, Biophysical Journal 104, 682a (2013).


A Cre-dependent GCaMP3 reporter mouse for neuronal imaging in vivo. H.A. Zariwala, B. G. Borghuis, T. M. Hoogland, L. Madisen, L. Tian, C. I. De Zeeuw, H. Zeng, L. L. Looger, K. Svoboda, and T.-W. Chen, The Journal of Neuroscience 32, 3131-3141 (2012).

Activity in motor-sensory projections reveals distributed coding in somatosensation L. Petreanu, D. A. Gutnisky, D. Huber, N.-l. Xu, D. H. O‚ Connor, L. Tian, L. L. Looger, and K. Svoboda, Nature 489, 299-303 (2012).

Engineering and application of genetically encoded calcium indicators. J. Akerboom, L. Tian, J. Marvin, & L.L. Looger, Genetically Encoded Functional Indicators 125-147 (2012).

Imaging neuronal activity with genetically encoded calcium indicators. L. Tian, S.A. Hires, & L.L. Looger, Cold Spring Harbor Protocols (2012)

Multiple dynamic representations in the motor cortex during sensorimotor learning. D. C. Huber, D. A. Gutnisky, S. Peron, D. H. O‚ Connor, J. S. Wiegert, L. Tian, T. G. Oertner, L. L. Looger, and K. Svoboda, Nature 484, 473-478 (2012).

Optimization of a GCaMP calcium indicator for neural activity imaging. J. Akerboom, T.-W. Chen, T. J. Wardill, L. Tian et al. The Journal of neuroscience 32, 13819-13840 (2012).

Selective esterase-ester pair for targeting small molecules with cellular specificity. L. Tian, Y. Yang, L. M. Wysocki, A. C. Arnold, A. Hu, B. Ravichandran, S. M. Sternson, L. L. Looger, and L. D. Lavis, Proceedings of the National Academy of Sciences 109, 4756-4761 (2012).


Imaging light responses of targeted neuron populations in the rodent retina. B.G. Borghuis, L. Tian, Y. Xu, S. S. Nikonov, N. Vardi, B. V. Zemelman, and L. L. Looger, The Journal of Neuroscience 31, 2855-2867 (2011).


Functional imaging of hippocampal place cells at cellular resolution during virtual navigation. D. A. Dombeck, C. D. Harvey, L. Tian, L. Looger, & D. W. Tank, Nature neuroscience 13, 1433-1440 (2010).

Toward the second generation of optogenetic tools. T. Knopfel, M. Z. Lin, A. Levskaya, L. Tian, J. Y. Lin, and E. S. Boyden, The Journal of Neuroscience 30, 14998-15004 (2010).


Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design.
J. Akerboom, J. D. V. Rivera, M. M. R. Guilbe, L. Tian, S. A. Hires, J. S. Marvin, L. L. Looger, and E. R. Schreiter, Journal of biological chemistry 284, 6455-6464 (2009).

Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. L. Tian, S. A. Hires, T. Mao, D. Huber, M. E. Chiappe, S. H. Chalasani, L. Petreanu, J. Akerboom, S. A. McKinney, E. R. Schreiter, C. I. Bargmann, V. Jayaraman, K. Svoboda, L. Looger, Nature methods 6, 875-881 (2009).


Genetically encoded fluorescent sensors for studying healthy and diseased nervous systems. L. Tian, & L. L. Looger, Drug Discovery Today: Disease Models 5, 27-35 (2008).

Reporting neural activity with genetically encoded calcium indicators. S. A. Hires, L. Tian, & L. L. Looger, Brain cell biology 36, 69-86 (2008).


Where to start and when to stop. L. Tian, & Matouschek, A., Nature structural & molecular biology 13, 668-670 (2006).


A conserved processing mechanism regulates the activity of transcription factors Cubitus interruptus and NF-kB. L. Tian, R. A. Holmgren, & A. Matouschek, Nature structural & molecular biology 12, 1045-1053 (2005).


An unstructured initiation site is required for efficient proteasome-mediated degradation. S. Prakash, L. Tian, L., K. S. Ratliff, R. E. Lehotzky, & A. Matouschek, Nature structural & molecular biology 11, 830-837 (2004).

Protein unfolding by the proteasome. A. Matouschek, S. Prakash, L. Tian, & Mensah, K., FASEB JOURNAL 18(8), C308-C308 (2004).


Olfactory identification and apolipoprotein E ε4 allele in mild cognitive impairment. Q. Wang, L. Tian, Y. Huang, Q. Song, L. He, J. N. Zhou, Brain research 951, 77-81 (2002).